HealthGate Document


Title
Single strand breaks in CHO cell DNA induced by ultrasonic cavitation in vitro.
Author
Miller DL; Thomas RM; Frazier ME
Address
Biology and Chemistry Department, Battelle Pacific Northwest Laboratories, Richland, WA 99352.
Source
Ultrasound Med Biol, 1991, 17:4, 401-6
Abstract
Ultrasonic cavitation induces a multiplicity of bioeffects in cell suspensions exposed in a 72 RPM rotating-tube exposure system. Single strand DNA breaks (SSBs) were found in cultured Chinese hamster ovary (CHO) cells exposed directly to 1.61 MHz ultrasound at a continuous 8 W/cm2 spatial peak temporal average (SPTA) intensity with cavitation for 10 min at 2 degrees C. Viability assessed by the trypan blue test was less than 1%, which indicates that these SSBs were in dead cells. Burst mode exposure with 10.5 microseconds bursts repeated each 21 microseconds not only caused SSBs at 5.6 W/cm2 and 8 W/cm2 (SPTA) for 10 min, but also allowed 20% and 7% viability, respectively. In order to determine if any of these breaks resided in the viable fraction of cells, the exposures were repeated with a 30 min postexposure incubation period at 37 degrees C to allow breaks in viable cells to repair. No significant repair occurred, relative to the samples which remained at 2 degrees C to prevent repair. A similar result was obtained with 10.5 microseconds bursts repeated each 42 microseconds at 4 W/cm2 (SPTA) with 46% viability. Thus, the observed ultrasonically induced SSBs reside primarily in the nonviable fraction of cells.
Language of Publication
English
Unique Identifier
92056203


MeSH Heading (Major)
DNA Damage|*; DNA, Single-Stranded|RE/*UL; Ultrasonics|*
MeSH Heading
Animal; Cell Survival|RE; Cells, Cultured; Cobalt Radioisotopes; Cold; Cricetulus; DNA Repair; Female; Gamma Rays; Hamsters; Heat; Ovary; Support, U.S. Gov't, P.H.S.; Time Factors

Publication Type
JOURNAL ARTICLE
ISSN
0301-5629
Country of Publication
ENGLAND
CAS Registry/EC Number
0 (Cobalt Radioisotopes); 0 (DNA, Single-Stranded)